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Sapienza University of Rome

9 ARTICLES PUBLISHED IN JoVE

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Education

Measuring Neuromuscular Junction Functionality
Emanuele Rizzuto 1, Simona Pisu 2, Carmine Nicoletti 2, Zaccaria Del Prete 1,3, Antonio Musarò 2,3
1Department of Mechanical and Aerospace Engineering, Sapienza University of Rome, 2Institute Pasteur Cenci-Bolognetti, DAHFMO-Unit of Histology and Medical Embryology, Sapienza University of Rome, 3Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia

A functional assessment of the neuromuscular junction (NMJ) can provide essential information on the communication between muscle and nerve. Here we describe a protocol to comprehensively evaluate both the NMJ and muscle functionality using two different muscle-nerve preparations, i.e. soleus-sciatic and diaphragm-phrenic.

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Neuroscience

Electroencephalographic, Heart Rate, and Galvanic Skin Response Assessment for an Advertising Perception Study: Application to Antismoking Public Service Announcements
Giulia Cartocci 1, Myriam Caratù 2, Enrica Modica 3, Anton Giulio Maglione 1, Dario Rossi 3, Patrizia Cherubino 4, Fabio Babiloni 1
1Department of Molecular Medicine, Sapienza University of Rome, 2Department of Communication and Social Research, Sapienza University of Rome, 3Department of Anatomical, Histological, Forensic, and Orthopedic Sciences, Sapienza University of Rome, 4BrainSigns SRL

The following protocol describes a series of operational and computational steps required to properly estimate the emotional and cerebral reaction of a group of subjects towards a selected number of Public Service Announcements (PSAs) against smoking, aired in the USA and Europe during the period between 1998 and 2015.

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Medicine

Regenerative Therapy by Suprachoroidal Cell Autograft in Dry Age-related Macular Degeneration: Preliminary In Vivo Report
Paolo Giuseppe Limoli 1, Enzo Maria Vingolo 2, Celeste Limoli 1, Sergio Zaccaria Scalinci 3, Marcella Nebbioso 4
1Low Vision Research Centre of Milan, 2Department of Ophthalmology, A. Fiorini Hospital, Sapienza University of Rome, 3Glaucoma and Low Vision Study Center, Department of General Surgery and Organ Transplants, University of Bologna, 4Department of Sense Organs, Faculty of Medicine and Odontology, Sapienza University of Rome

The goal of this study is to assess whether the suprachoroidal graft of adipose-derived stem cells included in the stromal vascular fraction and platelets derived from platelet-rich plasma by the Limoli Retinal Restoration Technique can improve visual acuity and retinal sensitivity responses in eyes affected by dry age-related macular degeneration.

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Immunology and Infection

A Novel In Vitro Wound Healing Assay to Evaluate Cell Migration
Floriana Cappiello 1, Bruno Casciaro 1, Maria Luisa Mangoni 1
1Department of Biochemical Sciences, Sapienza University of Rome

Here, we present a protocol to evaluate the effect of peptides on the migration of bronchial epithelial cells. This method allows for the rapid and highly reproducible obtainment of quantitative data on the speed of cell migration and wound closure.

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Developmental Biology

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
Stefano Martellucci 1,2, Costantino Santacroce 1, Valeria Manganelli 2, Francesca Santilli 1,2, Luca Piccoli 3, Michele Cassetta 3, Roberta Misasi 2, Maurizio Sorice 2, Vincenzo Mattei 1,2
1Laboratory of Experimental Medicine and Environmental Pathology, Sabina Universitas - Rieti University Hub, 2Department of Experimental Medicine, Sapienza University of Rome, 3Department of Science Dentistry and Maxillofacial, Sapienza University of Rome

Here we present a protocol for human Dental Pulp Stem Cells isolation and propagation in order to evaluate the prion protein expression during neuronal differentiation process.

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Developmental Biology

Human Blastocyst Biopsy and Vitrification
Roberta Maggiulli 1, Adriano Giancani 1,2, Danilo Cimadomo 1, Filippo M. Ubaldi 1, Laura Rienzi 1
1G.EN.E.R.A. Centers for Reproductive Medicine, 2DAHFMO, Unit of Histology and Medical Embryology, Sapienza University of Rome

Blastocyst biopsy and vitrification are required to efficiently conduct preimplantation genetic testing. An approach entailing the sequential opening of the zona pellucida and retrieval of 7-8 trophectoderm cells in day 5-7 post-insemination limits both the number of manipulations required and the exposure of the embryo to sub-optimal environmental conditions.

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Medicine

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
Roberta Maggiulli 1, Alberto Vaiarelli 1, Danilo Cimadomo 1, Adriano Giancani 1, Luisa Tacconi 1, Gemma Fabozzi 1, Filippo Maria Ubaldi 1, Laura Rienzi 1
1Clinica Valle Giulia, GeneraLife IVF

Oocyte cryopreservation is recognized by several international scientific societies as the gold standard for fertility preservation in postpubertal women. Appropriate clinical and laboratory strategies ensure maximum efficacy, efficiency, and safety of fertility preservation treatments.

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Chemistry

Phase Change Dimethyldioctadecylammonium-Shelled Microdroplets as a Promising Drug Delivery System: Results on 3D Spheroids of Mammalian Tumor Cells
Elisabetta Tortorella *1,2, Damiano Palmieri *1, Martina Piermarini 1, Daniele Gigante 1, Letizia Oddo 1, Yosra Toumia 1, Gaio Paradossi 1, Fabio Domenici 1
1Department of Chemical Sciences and Technologies, University of Rome Tor Vergata, 2Department of Molecular Medicine, Sapienza University of Rome

Decafluoropentane microdroplets developed with a shell of dimethyldioctadecylammonium bromide exhibited an exceptional colloidal stability and an actractive biointerface. DDAB-MDs proved to be efficient drug reservoirs characterized by a high affinity to plasma membranes together with enhanced uptake and antitumor activity of Doxorubicin against human triple-negative breast cancer (MDA-MB-231) 3D model.

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Biochemistry

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA
Núria Crua Asensio 1, Stefano Rizzieri 2, Alessandro Cuomo 2, Gian Gaetano Tartaglia 1,3, Elsa Zacco 1
1Center for Human Technology, Italian Institute of Technology (IIT), 2Department of Experimental Oncology, European Institute of Oncology (IEO), 3Department of Biology and Biotechnologies ‘Charles Darwin’, Sapienza University of Rome

Here, we present an optimized in vitro method to uncover, quantify, and validate protein interactors of specific RNA sequences, using total protein extract from human cells, streptavidin beads coated with biotinylated RNA, and mass spectrometry analysis.

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