Montpellier University

2 ARTICLES PUBLISHED IN JoVE

Biology

MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells

Brice Nzigou Mombo¹, Sabine Gerbal-Chaloin¹, Aleksandra Bokus¹, Martine Daujat-Chavanieu¹, Christian Jorgensen¹, Jean-Philippe Hugnot², Marie-Luce Vignais¹

¹Institute for Regenerative Medicine and Biotherapy (IRMB), INSERM U1183, Montpellier University, ²Institute of Neurosciences of Montpellier (INM), INSERM U1051, Montpellier University

Here, a protocol (MitoCeption) is presented to transfer mitochondria, isolated from human mesenchymal stem cells (MSC), to glioblastoma stem cells (GSC), with the goal of studying their biological effects on GSC metabolism and functions. A similar protocol can be adapted to transfer mitochondria between other cell types.

Cancer Research

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research

Guillaume Belthier^*1, Zeinab Homayed^*1, Céline Bouclier¹, Muriel Asari¹, Julie Pannequin¹

¹Department of Physiology and Cancer, Institute for Functional Genomics, Montpellier University

Culturing CTCs allows a deeper functional characterization of cancer, through assaying specific marker expression, and assessing drug resistance and the ability to colonize the liver among other possibilities. Overall, CTC culture could be a promising clinical tool for personalized medicine to improve patient outcome.