Inselspital, Bern University Hospital

3 ARTICLES PUBLISHED IN JoVE

JoVE Core

High-resolution Respirometry to Assess Mitochondrial Function in Permeabilized and Intact Cells

¹Department of Intensive Care Medicine, Inselspital, Bern University Hospital, University of Bern

High-resolution respirometry is used to determine mitochondrial oxygen consumption. This is a straightforward technique to determine mitochondrial respiratory chain complexes' (I-IV) respiratory rates, maximal mitochondrial electron transport system capacity, and mitochondrial outer membrane integrity.

Biology

Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements

Siamak Djafarzadeh¹, Stephan Mathias Jakob¹

¹Department of Intensive Care Medicine, Inselspital, Bern University Hospital

Here, a quadriceps muscle specimen is taken from an anaesthetized pig and mitochondria are isolated by differential centrifugation. Then, the respiratory rates of mitochondrial respiratory chain complexes I, II and IV are determined using high-resolution respirometry.

Medicine

Robotic Cochlear Implantation for Direct Cochlear Access

Marco Caversaccio^1,2, Georgios Mantokoudis¹, Franca Wagner³, Philipp Aebischer^1,2, Stefan Weder^1,2, Wilhelm Wimmer^1,2

¹Department of Otorhinolaryngology, Head and Neck Surgery, Inselspital, Bern University Hospital, University of Bern, ²Hearing Research Laboratory, ARTORG Center for Biomedical Engineering Research, University of Bern, ³Department of Diagnostic and Interventional Neuroradiology, Inselspital, Bern University Hospital

Robotic cochlear implantation is a procedure for minimally invasive inner ear access. Compared to conventional surgery, robotic cochlear implantation involves additional steps that need to be carried out in the operating room. In this article we give a description of the procedure and highlight the important aspects of robotic cochlear implantation.