Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical CharacterizationMandi J. Corenblum 1, Lalitha Madhavan 1
1Department of Neurology, The University of Arizona
Regular characterization of induced pluripotent stem cells (iPSCs), to ascertain maintenance of their pluripotent state, is an important step before these cells are used for other applications. Here we describe a method for the small-scale propagation of human iPSCs specifically designed to enable their easy and routine characterization via immunocytochemistry.