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The University of Arizona

1 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization
Mandi J. Corenblum 1, Lalitha Madhavan 1
1Department of Neurology, The University of Arizona

Regular characterization of induced pluripotent stem cells (iPSCs), to ascertain maintenance of their pluripotent state, is an important step before these cells are used for other applications. Here we describe a method for the small-scale propagation of human iPSCs specifically designed to enable their easy and routine characterization via immunocytochemistry.

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