Eberhard Karls University Tübingen

3 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Non-invasive In Vivo Fluorescence Optical Imaging of Inflammatory MMP Activity Using an Activatable Fluorescent Imaging Agent

Johannes Schwenck^1,2, Florian C. Maier¹, Manfred Kneilling^1,3, Stefan Wiehr¹, Kerstin Fuchs¹

¹Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University of Tübingen, ²Department of Nuclear Medicine, Eberhard Karls University of Tübingen, ³Department of Dermatology, Eberhard Karls University of Tübingen

This paper explains the application of fluorescent imaging using an activatable optical imaging probe to visualize the in vivo activity of key matrix metalloproteinases in two different experimental models of inflammation.

Immunology and Infection

Murine Lymphocyte Labeling by ⁶⁴Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Sabrina H. L. Hoffmann¹, Andreas Maurer¹, Dorothea I. Reck¹, Gerald Reischl¹, Bernd J. Pichler¹, Manfred Kneilling^1,2, Christoph M. Griessinger¹

¹Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, Eberhard Karls University Tübingen, ²Department of Dermatology, Eberhard Karls University Tübingen

Following the preparation of a ⁶⁴Cu-modified monoclonal antibody binding to a transgenic murine T cell receptor, T cells are radiolabeled in vivo, analyzed for viability, functionality, labeling stability and apoptosis, and adoptively transferred into mice with an airway delayed-type hypersensitivity reaction for non-invasive imaging by positron emission tomography/computed tomography (PET/CT).

Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts

Carmen J. Pastor-Maldonado¹, Tassula Proikas-Cezanne¹

¹Interfaculty Institute of Cell Biology, Eberhard Karls University Tübingen

This protocol provides detailed instructions for performing high-throughput, immunofluorescence-based ferritinophagy assessments in primary, skin-derived human fibroblasts.