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CSIR-National Chemical Laboratory

2 ARTICLES PUBLISHED IN JoVE

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Environment

Development of Metarhizium anisopliae as a Mycoinsecticide: From Isolation to Field Performance
Santosh G. Tupe 1, Ejaj K. Pathan 1, Mukund V. Deshpande 1
1Biochemical Sciences Division, CSIR-National Chemical Laboratory

Here, we report the different stages involved in the knowledge-based development of an effective mycoinsecticide, including the isolation, identification, screening, and selection of the "best-fit" entomopathogenic fungus, Metarhizium anisopliae, for the control of insect pests in agriculture.

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Biology

Developing a Feeding Assay System for Evaluating the Insecticidal Effect of Phytochemicals on Helicoverpa armigera
Sharada D. Mohite 1,2, Yogita P. Patil 1,2, Vikram J. Nichit 1,2, Rakesh S. Joshi 1,2
1Biochemical Sciences Division, CSIR-National Chemical Laboratory, 2Academy of Scientific and Innovative Research (AcSIR)

This protocol describes the obligate feeding assay to evaluate the potentially toxic effect of a phytochemical on the lepidopteran insect larvae. This is a highly scalable insect bioassay, easy to optimize the sublethal and lethal dose, deterrent activity, and physiological effect. This could be used for screening eco-friendly insecticides.

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