Evaporation-reducing Culture Condition Increases the Reproducibility of Multicellular Spheroid Formation in Microtiter PlatesViswanath Das 1, Tomáš Fürst 1, Soňa Gurská 1, Petr Džubák 1, Marián Hajdúch 1
1Institute of Molecular and Translational Medicine, Palacky University in Olomouc
The uneven loss of medium from microtiter plates affects the reproducibility of uniform multicellular tumor spheroid formation. Improving culture conditions to reduce significant medium loss will improve the reproducibility of spheroid formation and the results of spheroid-based assays using the liquid-overlay technique.
