Johns Hopkins Hospital

4 ARTICLES PUBLISHED IN JoVE

Medicine

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

Yong-Ung Lee¹, Tai Yi¹, Shuhei Tara¹, Avione Y. Lee¹, Narutoshi Hibino¹, Toshiharu Shinoka², Christopher K. Breuer^1,3

¹Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital, ²Department of Cardiothoracic Surgery, Nationwide Children's Hospital, ³Pediatric Surgery, Nationwide Children's Hospital

To improve our knowledge of cellular and molecular neotissue formation, a murine model of the TEVG was recently developed. The grafts were implanted as infrarenal vena cava interposition grafts in C57BL/6 mice. This model achieves similar results to those achieved in our clinical investigation, but over a far shortened time-course.

Medicine

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Yong-Ung Lee¹, Tai Yi¹, Iyore James¹, Shuhei Tara¹, Alexander J. Stuber¹, Kejal V. Shah¹, Avione Y. Lee¹, Tadahisa Sugiura¹, Narutoshi Hibino², Toshiharu Shinoka¹, Christopher K. Breuer^1,3

¹Tissue Engineering Program and Surgical Research, Nationwide Children's Hospital, ²Cardiothoracic Surgery, Nationwide Children's Hospital, ³Pediatric Surgery, Nationwide Children's Hospital

In order to understand the cellular and molecular mechanisms underlying neotissue formation and stenosis development in tissue engineered heart valves, a murine model of heterotopic heart valve transplantation was developed. A pulmonary heart valve was transplanted to recipient using the heterotopic heart transplantation technique.

Bioengineering

Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting

Chin Siang Ong^1,2, Takuma Fukunishi¹, Andrew Nashed¹, Adriana Blazeski³, Huaitao Zhang¹, Samantha Hardy¹, Deborah DiSilvestre², Luca Vricella¹, John Conte¹, Leslie Tung³, Gordon Tomaselli², Narutoshi Hibino¹

¹Division of Cardiac Surgery, Johns Hopkins Hospital, ²Division of Cardiology, Johns Hopkins Hospital, ³Department of Biomedical Engineering, Johns Hopkins University

This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials. 3D bioprinted cardiac patches exhibit mechanical integration of component spheroids and are highly promising in cardiac tissue regeneration and as 3D models of heart disease.

Bioengineering

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

Yang Bai^1,2, Enoch Yeung², Cecillia Lui², Chin Siang Ong², Isaree Pitaktong², Chenyu Huang², Takahiro Inoue², Hiroshi Matsushita², Chunye Ma², Narutoshi Hibino²

¹Department of Cardiac Surgery, The First Hospital of Jilin University, ²Division of Cardiac Surgery, Johns Hopkins Hospital

This protocol describes a net mold-based method to create three-dimensional scaffold-free cardiac tissues with satisfactory structural integrity and synchronous beating behavior.