Simultaneous Isolation of High Quality Cardiomyocytes, Endothelial Cells, and Fibroblasts from an Adult Rat HeartDursun Gündüz 1, Christian W. Hamm 1, Muhammad Aslam 1
1Internal Medicine, Cardiology and Angiology, University Hospital Giessen
Several protocols have been developed and described for the isolation of different cardiac cell types from a rat heart. Here, an optimized protocol is described that allows the isolation of high-quality major cardiac cell types (cardiomyocytes, endothelial cells, and fibroblasts) from a single preparation, reducing the experimental costs.
