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Hebrew University

3 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Electrophysiological Method for Whole-cell Voltage Clamp Recordings from Drosophila Photoreceptors
Ben Katz *1, Rita Gutorov *1, Elisheva Rhodes-Mordov 1, Roger C. Hardie 2, Baruch Minke 1
1Department of Medical Neurobiology, Faculty of Medicine and the Edmond and Lily Safra Center for Brain Sciences (ELSC), Hebrew University, 2Department of Physiology, Development and Neuroscience, University of Cambridge

Whole-cell recordings from Drosophila melanogaster photoreceptors enable the measurement of spontaneous dark bumps, quantum bumps, macroscopic responses to light, and current-voltage relationships under various conditions. In combination with D. melanogaster genetic manipulation tools, this method enables the study of the ubiquitous inositol-lipid signaling pathway and its target, the TRP channel.

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Neuroscience

Electrophysiological Methods for Measuring Photopigment Levels in Drosophila Photoreceptors
Rita Gutorov 1, Ben Katz 1, Baruch Minke 1
1Department of Medical Neurobiology, Faculty of Medicine and the Edmond and Lily Safra Center for Brain Sciences (ELSC), Hebrew University

We present a protocol to electrophysiologically characterize bi-stable photopigments: (i) exploiting the charge displacements within the photopigment molecules following photon-absorption and their huge amount in the photoreceptors, and (ii) exploiting the absorption-spectra differences of rhodopsin and metarhodopsin photopigment states. These protocols are useful to screen for mutations affecting bi-stable photopigment systems. 

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Methods for Studying the Optics, Physiology, and Biochemistry of the Fly Compound Eye
Ben Katz 1, Baruch Minke 1
1Department of Medical Neurobiology, Faculty of Medicine and the Edmond and Lily Safra Center for Brain Sciences (ELSC), Hebrew University

Methods for Studying the Optics, Physiology, and Biochemistry of the Fly Compound Eye

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