Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N6-threonylcarbamoyladenosine TcdA FunctionFrancisco J. Fernández 1,2,3, Sara Gómez 1, Sergio Navas-Yuste 1, Miguel López-Estepa 1, M. Cristina Vega 1
1Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC), Spanish National Research Council (CSIC), 2Department of Immunology, Complutense University School of Medicine, 3Abvance Biotech srl
A protocol is presented for the production of tRNA(UUU) and the analysis of tRNA(UUU) in complex with the enzyme TcdA by agarose gel retardation assays.
