Kanazawa University Graduate School of Medicine

2 ARTICLES PUBLISHED IN JoVE

Medicine

A Novel Method: Super-selective Adrenal Venous Sampling

Kohzoh Makita¹, Koshiro Nishimoto², Kanako Kiriyama-Kitamoto³, Shigehiro Karashima⁴, Tsugio Seki⁵, Masanori Yasuda⁶, Seishi Matsui⁷, Masao Omura³, Tetsuo Nishikawa³

¹Department of Radiology, Nerima Hikarigaoka Hospital, ²Department of Uro-Oncology, Saitama Medical University International Medical Center, ³Endocrinology & Diabetes Center, Yokohama Rosai Hospital, ⁴Division of Endocrinology and Metabolism, Kanazawa University Graduate School of Medicine, ⁵Department of Medical Education, School of Medicine, California University of Science and Medicine, ⁶Department of Pathology, Saitama Medical University International Medical Center, ⁷Department of Radiology, Yokohama Rosai Hospital

'Super-selective' adrenal venous sampling (ssAVS, also called segmental adrenal venous sampling: sAVS) was performed using a micro-catheter to identify adrenal segment(s) that produce excessive amounts of hormones. The ssAVS technique was described, cases in which adrenal segmental lesion(s) were identified by ssAVS were presented, and the usefulness of ssAVS in future adrenal research was discussed.

Biochemistry

Non-Radioactive In Vitro Cardiac Myosin Light Chain Kinase Assays

Kenta Kamikubo^*1, Osamu Tsukamoto^*1, Yuki Uyama-Saito^*1, Ryohei Oya¹, Tomoya Tsubota¹, Noboru Fujino³, Yoshihiro Asano², Hisakazu Kato¹, Ken Matsuoka¹, Seiji Takashima¹

¹Department of Medical Biochemistry, Osaka University Graduate School of Medicine/Frontier Biosciences, ²Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine/Frontier Biosciences, ³Department of Cardiovascular and internal medicine, Kanazawa University Graduate School of Medicine

This study describes protocols for nonradiometric methods, a bioluminescent ADP detection assay and a phosphate-affinity SDS-PAGE, to determine the kinase activity of cardiac myosin light chain kinase (cMLCK) and the phosphorylation level of its substrate, myosin regulatory light chain (MLC2v).