UMR5248 CNRS, Université de Bordeaux

1 ARTICLES PUBLISHED IN JoVE

JoVE Core

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Antoine Loquet¹, James Tolchard¹, Melanie Berbon¹, Denis Martinez¹, Birgit Habenstein¹

¹Institute of Chemistry, Biology of Membranes, Nanoobjects, UMR5248 CNRS, Université de Bordeaux

Structures of supramolecular protein assemblies at atomic resolution are of high relevance because of their crucial roles in a variety of biological phenomena. Herein, we present a protocol to perform high-resolution structural studies on insoluble and non-crystalline macromolecular protein assemblies by magic-angle spinning solid-state nuclear magnetic resonance spectroscopy (MAS SSNMR).