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5 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
Naoki Ihara 1, Yuji Ikegaya 1,2, Haruki Takeuchi 1,3
1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Center for Information and Neural Networks, National Institute of Information and Communications Technology, 3PRESTO, Japan Science and Technology Agency (JST)

Olfactory sensory neurons express a wide variety of axon-sorting molecules to establish proper neural circuitry. This protocol describes an immunohistochemical staining method to visualize combinatorial expressions of axon-sorting molecules at the axon termini of olfactory sensory neurons.

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Neuroscience

Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat
Yu Shikano 1, Takuya Sasaki 1, Yuji Ikegaya 1,2
1Graduate School of Pharmaceutical Sciences, The University of Tokyo, 2Center for Information and Neural Networks

This study introduces a method for the simultaneous recording of local field potentials in the brain, electrocardiograms, electromyograms, and breathing signals of a freely moving rat. This technique, which reduces experimental costs and simplifies data analysis, will contribute to the understanding of the interactions between the brain and peripheral organs.

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Biology

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon
Atsushi Matsuda 1,2, Takako Koujin 1, Lothar Schermelleh 3, Tokuko Haraguchi 1,2, Yasushi Hiraoka 1,2
1Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, 2Graduate School of Frontier Biosciences, Osaka University, 3Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford

Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software, Chromagnon.

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Behavior

Tactile Semiautomatic Passive-Finger Angle Stimulator (TSPAS)
Wu Wang 1, Jiajia Yang 2,4, Yinghua Yu 2,3,4, Qiong Wu 5,2, Satoshi Takahashi 2, Yoshimichi Ejima 2, Jinglong Wu 6,2
1Cognitive Neuroscience Laboratory, Graduate School of Natural Science and Technology, Okayama University, 2Cognitive Neuroscience Laboratory, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, 3Center for Information and Neural Networks, National Institute of Information and Communications Technology, 4Section on Functional Imaging Methods, National Institute of Mental Health, 5School of Education, Suzhou University of Science and Technology, 6Beijing Institute of Technology

Presented is the tactile semiautomated passive-finger angle stimulator TSPAS, a new way to assess tactile spatial acuity and tactile angle discrimination using a computer-controlled tactile stimulus system that applies raised angle stimuli to a subject's passive fingerpad, while controlling for movement speed, distance, and contact duration.

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Neuroscience

Reliable Acquisition of Electroencephalography Data during Simultaneous Electroencephalography and Functional MRI
Hui Ming Khoo 1, Yuya Fujita 1, Naoki Tani 1, Tetsuya Shimokawa 2, Natalja Zazubovits 3, Satoru Oshino 1, Jean Gotman 3, Haruhiko Kishima 1
1Department of Neurosurgery, Osaka University Graduate School of Medicine, 2Center for Information and Neural Networks, National Institute of Information and Communications Technology, 3Montreal Neurological Institute and Hospital, McGill University

This article provides a straightforward protocol for acquiring good quality electroencephalography (EEG) data during simultaneous EEG and functional magnetic resonance imaging by utilizing readily available medical products.

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