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University of Modena and Reggio Emilia

4 ARTICLES PUBLISHED IN JoVE

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Biology

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
Francesco Castagnetti 1,2, Elisabetta Fiacco 3, Carol Imbriano 4, Lucia Latella 1,2
1Department of Medicine, Institute of Translational Pharmacology, Italian National Research Council, 2Epigenetics and Regenerative Medicine, IRCCS Fondazione Santa Lucia, 3Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), 4Department of Life Sciences, University of Modena and Reggio Emilia

Active autophagy is associated with productive muscle regeneration, which is essential for Muscle Stem Cell (MuSC) activation. Here, we provide a protocol for the in situ detection of LC3, an autophagy marker in MyoD-positive MuSCs of muscle tissue sections from control and injured mice.

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Genetics

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
Oriana Romano 1,2, Ingrid Cifola 3, Valentina Poletti 4, Marco Severgnini 3, Clelia Peano 3, Gianluca De Bellis 3, Fulvio Mavilio 1,4, Annarita Miccio 1,2,4,5
1Center for Genome Research, Department of Life Sciences, University of Modena and Reggio Emilia, 2Laboratory of Chromatin and Gene Regulation During Development, Imagine Institute, 3Institute for Biomedical Technologies, CNR, 4Généthon, 5Sorbonne Paris Cité - Université Paris Descartes

Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.

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Biology

An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
Daniela Benati 1, Fabienne Cocchiarella 1, Alessandra Recchia 1
1Centre for Regenerative Medicine, Department of Life Sciences, University of Modena and Reggio Emilia

This protocol describes a method to achieve stable integration of a gene of interest into the human genome by the transcriptionally regulated Sleeping Beauty system. Preparation of the integration of defective lentiviral vectors, the in vitro transduction of human cells, and the molecular assay on transduced cells are reported.

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Medicine

Platelet-Rich Plasma Lysate for Treatment of Eye Surface Diseases
Lucia Merolle 1, Barbara Iotti 1, Pamela Berni 1, Elisa Bedeschi 1, Katya Boito 1, Eleonora Maurizi 2, Gaia Gavioli 1, Agnese Razzoli 1,3, Roberto Baricchi 1, Chiara Marraccini 1, Davide Schiroli 1
1Transfusion Medicine Unit, AUSL-IRCCS di Reggio Emilia, 2Interdepartmental Centre for Regenerative Medicine “Stefano Ferrari”, University of Modena and Reggio Emilia, 3Clinical and Experimental Medicine PhD Program, University of Modena and Reggio Emilia

Platelet lysates represent an emerging tool for the treatment of ocular surface diseases. Here, we propose a method for the preparation, dispensation, storage, and characterization of platelet lysate collected from platelet donors.

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