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CRCHU de Québec: L’Hôtel-Dieu de Québec

1 ARTICLES PUBLISHED IN JoVE

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Biochemistry

Quantitative Immunofluorescence to Measure Global Localized Translation
Jonathan Bergeman 1, Marc-Étienne Huot 1,2
1Centre de Recherche sur le Cancer de l’Université Laval, Faculté de Médecine, Département de Biologie moléculaire, biochimie médicale et pathologie, Université Laval, 2CRCHU de Québec: L’Hôtel-Dieu de Québec

This manuscript describes a method to visualize and quantify localized translation events in subcellular compartments. The approach proposed in this manuscript requires a basic confocal imaging system and reagents and is rapid and cost-effective.

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