Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry MeasurementThomas Laumonier *1, Stéphane Koenig *2, Sophie Saüc 2,3, Maud Frieden 3
1Department of Orthopedic Surgery, Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences, Geneva Medical Center, 3Department of Cell Physiology and Metabolism, Geneva Medical Center
Here, we describe a methodology to obtain a pure population of human myoblasts from adult muscle tissue. These cells are used to study in vitro skeletal muscle differentiation and, in particular, to study proteins involved in Ca2+ signaling.
