Imagine Institute

1 ARTICLES PUBLISHED IN JoVE

Genetics

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

Oriana Romano^1,2, Ingrid Cifola³, Valentina Poletti⁴, Marco Severgnini³, Clelia Peano³, Gianluca De Bellis³, Fulvio Mavilio^1,4, Annarita Miccio^1,2,4,5

¹Center for Genome Research, Department of Life Sciences, University of Modena and Reggio Emilia, ²Laboratory of Chromatin and Gene Regulation During Development, Imagine Institute, ³Institute for Biomedical Technologies, CNR, ⁴Généthon, ⁵Sorbonne Paris Cité - Université Paris Descartes

Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.