Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory RegionsOriana Romano 1,2, Ingrid Cifola 3, Valentina Poletti 4, Marco Severgnini 3, Clelia Peano 3, Gianluca De Bellis 3, Fulvio Mavilio 1,4, Annarita Miccio 1,2,4,5
1Center for Genome Research, Department of Life Sciences, University of Modena and Reggio Emilia, 2Laboratory of Chromatin and Gene Regulation During Development, Imagine Institute, 3Institute for Biomedical Technologies, CNR, 4Généthon, 5Sorbonne Paris Cité - Université Paris Descartes
Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.