Agriculture & Agri-Food Canada

4 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Ziming Cheng¹, Ting Zhou^1,2, Azhar Merchant¹, Thomas J. Prihoda³, Brian L. Wickes^4,5, Guogang Xu², Christi A. Walter^2,5, Vivienne I. Rebel^1,2,5

¹Greehey Children's Cancer Research Institute, UT Health Science Center at San Antonio, ²Department of Cellular and Structural Biology, UT Health Science Center at San Antonio, ³Department of Pathology, UT Health Science Center at San Antonio, ⁴Department of Microbiology, UT Health Science Center at San Antonio, ⁵Cancer Therapy and Research Center, UT Health Science Center at San Antonio

Here we describe an in vivo mutagenesis assay for small numbers of purified hematopoietic cells using the LacI transgenic mouse model. The LacI gene can be isolated to determine the frequency, location, and type of DNA mutants spontaneously arisen or after exposure to genotoxins.

JoVE Journal

A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling

Naeem Nathoo^1,2, Mark A. Bernards², Jacqueline MacDonald³, Ze-Chun Yuan^1,3

¹London Research and Development Centre, Agriculture & Agri-Food Canada, ²Department of Biology, University of Western Ontario, ³Department of Microbiology and Immunology, University of Western Ontario

The described hydroponic cocultivation system supports intact plants with metal mesh screens and cocultivates them with bacteria. Plant tissue, bacteria, and secreted molecules can then be separately harvested for downstream analyses, simultaneously allowing for the molecular responses of both plant hosts and interacting microbes or microbiomes to be investigated.

Behavior

Combining Quantitative Food-intake Assays and Forcibly Activating Neurons to Study Appetite in Drosophila

Lifen Jiang^*1,2, Yinpeng Zhan^*2,3, Yan Zhu^2,3

¹School of Life Science, University of Science and Technology of China, ²State key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, ³University of Chinese Academy of Sciences

Quantitative food-intake assays with dyed food provide a robust and high-throughput means to evaluate feeding motivation. Combining the food consumption assay with thermogenetic and optogenetic screens is a powerful approach to investigate the neural circuits underlying appetite in adult Drosophila melanogaster.

Analysis of Microbial Metabolites of Deoxynivalenol Through an Immuno-Affinity Enrichment Method

Yan Zhu^*1, Yousef I. Hassan^*1, Chenggang Cai², Xiu-Zhen Li¹, Suqin Shao¹, Ting Zhou¹

¹Guelph Research and Development Center, Agriculture & Agri-Food Canada, ²School of Biological and Chemical Engineering, Zhejiang University of Science and Technology

The presented work highlights the development of a novel and efficient protocol of a high-performance liquid chromatography approach coupled with an immuno-affinity enrichment step to detect and quantify multiple microbial metabolites of deoxynivalenol (DON), namely 3-epi-DON, 3-keto-DON, and deepoxy-DON (DOM-1).