Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering

3 ARTICLES PUBLISHED IN JoVE

Environment

Preparation of Authigenic Pyrite from Methane-bearing Sediments for In Situ Sulfur Isotope Analysis Using SIMS

Zhiyong Lin^1,3, Xiaoming Sun^1,2,3,4, Jörn Peckmann⁵, Yang Lu^2,3, Harald Strauss⁶, Li Xu^2,3, Hongfeng Lu⁷, Barbara M.A. Teichert⁶

¹School of Earth Sciences and Engineering, Sun Yat-sen University, ²School of Marine Sciences, Sun Yat-sen University, ³Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, ⁴South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, ⁵Institut für Geologie, Universität Hamburg, ⁶Institut für Geologie und Paläontologie, Westfälische Wilhelms-Universität Münster, ⁷Guangzhou Marine Geological Survey

Analyses of the sulfur isotopic composition (δ³⁴S) of pyrite from methane-bearing sediments have typically focused on bulk samples. Here, we applied secondary ion mass spectroscopy to analyze the δ³⁴S values of various pyrite generations to understand the diagenetic history of pyritization.

Medicine

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Li Xu¹, Yandi Gao¹, Yeh Siang Lau¹, Renzhi Han¹

¹Department of Surgery, Davis Heart and Lung Research Institute, Biomedical Sciences Graduate Program, Biophysics Graduate Program, Ohio State University Wexner Medical Center

Here we provide a detailed protocol to carry out in vivo cardiac gene editing in mice using recombinant Adeno-Associated Virus(rAAV)-mediated delivery of CRISPR. This protocol offers a promising therapeutic strategy to treat dystrophic cardiomyopathy in Duchenne muscular dystrophy and can be used to generate cardiac-specific knockout in postnatal mice.

Chemistry

Synthesis of Metal Nanoparticles Supported on Carbon Nanotube with Doped Co and N Atoms and its Catalytic Applications in Hydrogen Production

Pui-Ching Poon^*1, Ka-Man Lee^*1, Yuanhao Wang², William Wai Yan Lam¹, Peter Shu-Wai Leung¹, Xiao-Ying Lu¹, Weiqun Li³, B. Layla Mehdi³, Yang Lu⁴, Chi-Wing Tsang¹, Eugene Yin Cheung Wong²

¹Faculty of Science and Technology, Technological and Higher Education Institute of Hong Kong (THEi), ²Department of Supply Chain and Information Management, The Hang Seng University of Hong Kong, ³Department of Mechanical, Materials and Aerospace Engineering, University of Liverpool, ⁴Laboratory of Building Environment and New Energy Resources, School of Civil Engineering, Faculty of Infrastructure Engineering, Dalian University of Technology

Here, we present a protocol to synthesize Co nanoparticles supported on carbon nanotubes with Co- and N- dopants for hydrogen productions.