University of California-Davis

3 ARTICLES PUBLISHED IN JoVE

Behavior

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

Shani Leviatan Ben-Arye¹, Hai Yu², Xi Chen², Vered Padler-Karavani¹

¹Department of Cell Research and Immunology, Tel Aviv University, ²Department of Chemistry, University of California-Davis

A sialoglycan microarray assay can be used to evaluate anti-Neu5Gc antibodies in human sera, making it a potential high-throughput diagnostic assay for cancer and other chronic inflammation-mediated human diseases.

Cancer Research

Orthotopic Transplantation of Breast Tumors as Preclinical Models for Breast Cancer

Xiangdong Lv^*1,2,3, Lacey E. Dobrolecki^*1,2,3, Yao Ding^1,2,3, Jeffrey M. Rosen^1,2,3, Michael T. Lewis^1,2,3, Xi Chen^1,2,3

¹Department of Molecular and Cellular Biology, Baylor College of Medicine, ²Lester and Sue Smith Breast Center, Baylor College of Medicine, ³Dan L. Duncan Cancer Center, Baylor College of Medicine

Patient-derived xenograft (PDX) models and transplantable genetically engineered mouse models faithfully recapitulate human disease and are preferred models for basic and translational breast cancer research. Here, a method is described to orthotopically transplant breast tumor fragments into the mammary fat pad to study tumor biology and evaluate drug response.

Biology

Systematic Assessment of Mammalian Skull Specimens for Dental and Temporomandibular Joint Pathology

Janny Evenhuis¹, Boaz Arzi², Frank J. M. Verstraete²

¹Dentistry and Oral Surgery Service, William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California-Davis, ²Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California-Davis

The present protocol describes the techniques for the systematic assessment of skull specimens to characterize anatomical and developmental variations and abnormalities of the teeth, periodontal disease, endodontal disease, and temporomandibular joint pathology.