Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common BeanKalpana Nanjareddy *1, Manoj-Kumar Arthikala *1, Alma-Leticia Aguirre 1, Brenda-Mariana Gómez 1, Miguel Lara 2
1Ciencias Agrogenómicas, Escuela Nacional de Estudios Superiores Unidad León- Universidad Nacional Autónoma de México (UNAM), 2Instituto de Biología, Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, Coyoacan
Promoter expression analyses are crucial to improving the understanding of gene regulation and the spatiotemporal expression of target genes. Herein we present a protocol to identify, isolate, and clone a plant promoter. Further, we describe the characterization of the nodule-specific promoter in the common bean hairy roots.
