Hebei Province Key Research Office of Traditional Chinese Medicine Against Dementia

1 ARTICLES PUBLISHED IN JoVE

Behavior

Establishment of a Valuable Mimic of Alzheimer's Disease in Rat Animal Model by Intracerebroventricular Injection of Composited Amyloid Beta Protein

Wu Xiaoguang^*1,3,5, Cheng Jianjun^*1,3,4, Cao Qinying^*2, Zhang Hui^2,3, Yang Lukun^1,3,4, Shang Yazhen^1,3,4

¹Institute of Traditional Chinese Medicine, Chengde Medical College, ²Shijiazhuang Obstetrics and Gynecology Hospital, ³Hebei Province Key Research Office of Traditional Chinese Medicine Against Dementia, ⁴Hebei Province Key Laboratory of Traditional Chinese Medicine Research and Development, ⁵Institute of Basic Medical Research of Basic Medical School

This is a protocol to mimic Alzheimer's Disease in rats by evaluation of spatial memory impairment, neuronal pathological changes, neuronal amyloid beta protein (Aβ) burden, and neurofibrillary tangles aggregation, induced by the injection of Aβ25-35 combined with aluminum trichloride and recombinant human transforming growth factor-β1.