Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster BrainsSeth M. Kelly 1,2, Alexandra Elchert 3, Michael Kahl 2,3
1Program in Neuroscience, The College of Wooster, 2Department of Biology, The College of Wooster, 3Program in Biochemistry, Cellular, and Molecular Biology, The College of Wooster
This protocol describes the dissection and immunostaining of adult Drosophila melanogaster brain tissues. Specifically, this protocol highlights the use of Drosophila mushroom body and photoreceptor neurons as example neuronal subsets that can be accurately used to uncover general principles underlying many aspects of neuronal development.