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University of Valencia

5 ARTICLES PUBLISHED IN JoVE

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Biology

Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles
Estela Selma-Soriano 1,2,3, Rubén Artero 1,2,3, Beatriz Llamusi 1,2,3
1Department of Genetics and Interdisciplinary Research Structure for Biotechnology and Biomedicine (BIOTECMED), University of Valencia, 2Incliva Health Research Institute, 3Joint unit CIPF-Incliva

We report a method to quantify muscle area, which is an indirect method to determine muscle mass in Drosophila adults. We demonstrate the application of our methodology by analyzing the indirect flight muscles in a Drosophila model of Myotonic Dystrophy disease.

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Biology

Fish Sperm Assessment Using Software and Cooling Devices
Carina Caldeira 1, Carles Soler 1,2
1PROiSER R+D S.L., 2Department of Cellular Biology, Functional Biology and Physical Anthropology, University of Valencia

The present protocol describes a procedure of fish sperm assessment using computer-assisted sperm analysis and cooling devices. The software gives a rapid, accurate and quantitative analysis of fish sperm quality based on spermatozoa motility, which can be a useful tool in aquaculture to improve reproduction success.

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Environment

Data Collection on Marine Litter Ingestion in Sea Turtles and Thresholds for Good Environmental Status
Marco Matiddi 1, Giuseppe A. deLucia 2, Cecilia Silvestri 1, Gaëlle Darmon 3, Jesús Tomás 4, Christopher K. Pham 5, Andrea Camedda 2, Frederic Vandeperre 5,6, Françoise Claro 7, Yakup Kaska 8, Helen Kaberi 9, Ohiana Revuelta 4, Raffaella Piermarini 1, Roberto Daffina 1, Marco Pisapia 1, Daniela Genta 1, Doğan Sözbilen 8, Mohamed N. Bradai 10, Yasmina Rodríguez 5, Delphine Gambaiani 3, Catherine Tsangaris 9, Olfa Chaieb 10, Judicaëlle Moussier 7, Ana L. Loza 11, Claude Miaud 3, 13
1Italian National Institute for Environmental Protection and Research (ISPRA), 2Institute for Coastal Marine Environment-National Research Council (IAMC-CNR), 3EPHE, PSL Research University, UMR 5175 CE3FE, CNRS, UM, Univ P. Valery, SupAgro, IRD, INRA, Biogéographie et Écologie des Vertébrés, 4Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, 5Departamento de Oceanografia e Pescas, Instituto do Mar/Okeanos, Universidade dos Açores, 6MARE - Marine and Environmental Sciences Centre, Universidade dos Açores, 7Muséum national d' Histoire naturelle (MNHN), 8Sea Turtle Research and Application Center (DEKAMER), Pamukkale University, 9Institute of Oceanografy Hellenic Centre for Marine Research (HCMR), 10Institut National des Sciences et Technologies de la Mer (INSTM), 11University of Las Palmas de Gran Canaria, 12INDICIT consortium

The protocol focuses on the collection of sea turtle samples, describing all the steps from the animal recovery and necropsy to the classification and quantification of ingested marine litter. Moreover, the representative results show how to use the collected data to elaborate the possible thresholds for Good Environmental Status.

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Optimal Fixation Position of Deep Brain Stimulation Electrodes for Parkinson's Disease in Humans
Alfredo Rosado-Muñoz 1, Juan F. Guerrero-Martinez 1, Luciano Schiaffino 2, Manuel Bataller-Mompeán 1, Antonio Gutiérrez 3
1Group for Digital Design and Processing (GDDP), ETSE, Department of Electronic Engineering, University of Valencia, 2Laboratory of Rehabilitation Engineering, National University of Entre Ríos, 3Functional Neurosurgery Unit, La Fe Hospital

The fixation of deep brain stimulation microelectrodes for Parkinson’s disease requires brain surgery. This work describes a method to determine the optimal position of the stimulation electrodes during surgery, which requires a combination of techniques.

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Cancer Research

Enhancing Prostate Tumor Biobanking Reliability with Improved Sampling Technique and Histological Characterization
Eduardo Giner Moreno *1, Francisco Quereda-Flores *2, Josselyn A. Hernández Chinchilla *1, Raquel Amigo Moreno 3, María Dolores Perez Ruiz 1, Aileen Carcelén López 3, Jorge Rocher Benlloch 3, Pilar Molió Sanchís 3, Adrián Casares Calzada 3, María Ángeles Marqués-Torrejón 4, Conrado Martínez-Cadenas 4, José Luis Ruiz Cerdá 2,5, Alba Loras Monfort 4,5, David Ramos Soler 1,6
1Department of Pathology, Hospital Universitario y Politécnico La Fe, 2Department of Urology, Hospital Universitario y Politécnico La Fe, 3Biobanco La Fe, Instituto de Investigación Sanitaria La Fe, 4Department of Medicine, Jaume I University of Castellon, 5Department of Surgery, University of Valencia, 6Department of Pathology, University of Valencia

This protocol describes a method for facilitating the collection of samples from radical prostatectomy specimens. The goal is to map, characterize, and micro-macro dissect tissue samples from the specimens based on anatomopathological criteria before storing them in a Biobank.

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