University of Valencia

6 ARTICLES PUBLISHED IN JoVE

Biology

Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles

Estela Selma-Soriano^1,2,3, Rubén Artero^1,2,3, Beatriz Llamusi^1,2,3

¹Department of Genetics and Interdisciplinary Research Structure for Biotechnology and Biomedicine (BIOTECMED), University of Valencia, ²Incliva Health Research Institute, ³Joint unit CIPF-Incliva

We report a method to quantify muscle area, which is an indirect method to determine muscle mass in Drosophila adults. We demonstrate the application of our methodology by analyzing the indirect flight muscles in a Drosophila model of Myotonic Dystrophy disease.

Biology

Fish Sperm Assessment Using Software and Cooling Devices

Carina Caldeira¹, Carles Soler^1,2

¹PROiSER R+D S.L., ²Department of Cellular Biology, Functional Biology and Physical Anthropology, University of Valencia

The present protocol describes a procedure of fish sperm assessment using computer-assisted sperm analysis and cooling devices. The software gives a rapid, accurate and quantitative analysis of fish sperm quality based on spermatozoa motility, which can be a useful tool in aquaculture to improve reproduction success.

Environment

Data Collection on Marine Litter Ingestion in Sea Turtles and Thresholds for Good Environmental Status

Marco Matiddi¹, Giuseppe A. deLucia², Cecilia Silvestri¹, Gaëlle Darmon³, Jesús Tomás⁴, Christopher K. Pham⁵, Andrea Camedda², Frederic Vandeperre^5,6, Françoise Claro⁷, Yakup Kaska⁸, Helen Kaberi⁹, Ohiana Revuelta⁴, Raffaella Piermarini¹, Roberto Daffina¹, Marco Pisapia¹, Daniela Genta¹, Doğan Sözbilen⁸, Mohamed N. Bradai¹⁰, Yasmina Rodríguez⁵, Delphine Gambaiani³, Catherine Tsangaris⁹, Olfa Chaieb¹⁰, Judicaëlle Moussier⁷, Ana L. Loza¹¹, Claude Miaud³, ¹³

¹Italian National Institute for Environmental Protection and Research (ISPRA), ²Institute for Coastal Marine Environment-National Research Council (IAMC-CNR), ³EPHE, PSL Research University, UMR 5175 CE3FE, CNRS, UM, Univ P. Valery, SupAgro, IRD, INRA, Biogéographie et Écologie des Vertébrés, ⁴Cavanilles Institute of Biodiversity and Evolutionary Biology, University of Valencia, ⁵Departamento de Oceanografia e Pescas, Instituto do Mar/Okeanos, Universidade dos Açores, ⁶MARE - Marine and Environmental Sciences Centre, Universidade dos Açores, ⁷Muséum national d' Histoire naturelle (MNHN), ⁸Sea Turtle Research and Application Center (DEKAMER), Pamukkale University, ⁹Institute of Oceanografy Hellenic Centre for Marine Research (HCMR), ¹⁰Institut National des Sciences et Technologies de la Mer (INSTM), ¹¹University of Las Palmas de Gran Canaria, ¹²INDICIT consortium

The protocol focuses on the collection of sea turtle samples, describing all the steps from the animal recovery and necropsy to the classification and quantification of ingested marine litter. Moreover, the representative results show how to use the collected data to elaborate the possible thresholds for Good Environmental Status.

Optimal Fixation Position of Deep Brain Stimulation Electrodes for Parkinson's Disease in Humans

Alfredo Rosado-Muñoz¹, Juan F. Guerrero-Martinez¹, Luciano Schiaffino², Manuel Bataller-Mompeán¹, Antonio Gutiérrez³

¹Group for Digital Design and Processing (GDDP), ETSE, Department of Electronic Engineering, University of Valencia, ²Laboratory of Rehabilitation Engineering, National University of Entre Ríos, ³Functional Neurosurgery Unit, La Fe Hospital

The fixation of deep brain stimulation microelectrodes for Parkinson’s disease requires brain surgery. This work describes a method to determine the optimal position of the stimulation electrodes during surgery, which requires a combination of techniques.

Cancer Research

Enhancing Prostate Tumor Biobanking Reliability with Improved Sampling Technique and Histological Characterization

Eduardo Giner Moreno^*1, Francisco Quereda-Flores^*2, Josselyn A. Hernández Chinchilla^*1, Raquel Amigo Moreno³, María Dolores Perez Ruiz¹, Aileen Carcelén López³, Jorge Rocher Benlloch³, Pilar Molió Sanchís³, Adrián Casares Calzada³, María Ángeles Marqués-Torrejón⁴, Conrado Martínez-Cadenas⁴, José Luis Ruiz Cerdá^2,5, Alba Loras Monfort^4,5, David Ramos Soler^1,6

¹Department of Pathology, Hospital Universitario y Politécnico La Fe, ²Department of Urology, Hospital Universitario y Politécnico La Fe, ³Biobanco La Fe, Instituto de Investigación Sanitaria La Fe, ⁴Department of Medicine, Jaume I University of Castellon, ⁵Department of Surgery, University of Valencia, ⁶Department of Pathology, University of Valencia

This protocol describes a method for facilitating the collection of samples from radical prostatectomy specimens. The goal is to map, characterize, and micro-macro dissect tissue samples from the specimens based on anatomopathological criteria before storing them in a Biobank.

Chemistry

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Alfonso Narváez^1,2, Laura Gámiz-Gracia¹, Ana M. García-Campaña¹, Maykel Hernández-Mesa¹

¹Department of Analytical Chemistry, Faculty of Sciences, University of Granada, ²Department of Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine, Faculty of Pharmacy and Food Science, University of Valencia

This protocol presents a validated liquid chromatography-ion mobility-high resolution mass spectrometry method to determine the presence of ergot alkaloids in food in compliance with the recently released Commission Regulation (EU) 2023/915.