The Second Hospital of Jilin University

2 ARTICLES PUBLISHED IN JoVE

Medicine

Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis

Aoyuan Cui¹, Zhimin Hu¹, Yamei Han¹, Yi Yang², Yu Li¹

¹CAS Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Chinese Academy of Sciences, ²Suzhou Niumag Analytical Instrument Corporation

Here, optimized methods to generate in vivo and in vitro models of hepatic steatosis and to analyze the steatotic phenotypes and related physiological parameters are described.

Immunology and Infection

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

Cara L. Haymaker^*1, Yared Hailemichael^*1, Yi Yang^2,3, Roza Nurieva²

¹Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center, ²Department of Immunology, University of Texas MD Anderson Cancer Center, ³Department of Radiation Oncology, The Second Hospital of Jilin University

The goal of this protocol is to allow for detection of in vivo antigen-specific killing of a target cell in a murine model.