RenOVAte Biosciences Inc

2 ARTICLES PUBLISHED IN JoVE

Biology

Utero-tubal Embryo Transfer and Vasectomy in the Mouse Model

Pablo Bermejo-Alvarez^1,2, Ki-Eun Park^1,2, Bhanu P. Telugu^1,2

¹Animal Bioscience and Biotechnology Laboratory, United States Department of Agriculture, ²Department of Animal and Avian Sciences, University of Maryland

Utero-tubal embryo transfer uses the utero-tubal junction as a barrier to prevent the embryo outflow that may occur when performing uterine transfer. Vasectomized males are required to obtain pseudopregnant recipients for embryo transfer. Both techniques are discussed.

Bioengineering

Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System

Anjali Nandal^1,2, Barbara Mallon³, Bhanu P. Telugu^1,2,4

¹Department of Animal and Avian Sciences, University of Maryland, ²Animal Bioscience and Biotechnology Laboratory, ARS, USDA, ³NIH Stem Cell Unit, Bethesda, National Institutes of Health, ⁴RenOVAte Biosciences Inc

This protocol describes in detail the generation of footprint-free induced pluripotent stem cells (iPSCs) from human pancreatic cells in feeder-free conditions, followed by editing using CRISPR/Cas9 ribonucleoproteins and characterization of the modified single-cell clones.