Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma MansoniNathalie Dinguirard 1, Codie Heinemann 1, Timothy P. Yoshino 1
1Department of Pathobiological Sciences, University of Wisconsin, School of Veterinary Medicine
This article describes a protocol for the large-scale axenic isolation and collection of free-swimming miracidia of the human blood fluke Schistosoma mansoni and their subsequent processing for introduction into in vitro culture.
