Roche Innovation Center Basel

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

High-resolution Confocal Imaging of the Blood-brain Barrier: Imaging, 3D Reconstruction, and Quantification of Transcytosis

Roberto Villaseñor¹, Ludovic Collin¹

¹Roche Pharma Research and Early Development (pRED), Neuroimmunology, Roche Innovation Center Basel

Here we present a microscopy-based protocol for high-resolution imaging and a three-dimensional reconstruction of the mouse neurovascular unit and blood-brain barrier using brain free-floating sections. This method allows for the visualization, analysis, and quantification of intracellular organelles at the BBB.

Neuroscience

Methods for the Discovery of Novel Compounds Modulating a Gamma-Aminobutyric Acid Receptor Type A Neurotransmission

Frédéric Knoflach¹, Maria-Clemencia Hernandez¹, Daniel Bertrand²

¹Discovery Neuroscience, Pharma Research and Early Development, Roche Innovation Center Basel, ²HiQScreen Sàrl 6

Here, we present protocols to discover compounds active at GABA_A receptors, from the binding to the physiology and pharmacology.