Sign In

Universidad de Salamanca

1 ARTICLES PUBLISHED IN JoVE

image

Biochemistry

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
Myriam Jaraíz-Rodríguez 1, Ana González-Sánchez 1,2, Laura García-Vicente 1, Jose M. Medina 1, Arantxa Tabernero 1
1Instituto de Neurociencias de Castilla y León (INCYL), Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, 2Centre for Cancer Research & Cell Biology (CCRCB), School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast

This is a protocol to study intracellular protein-protein interactions based on the biotin-avidin pull-down system with the novelty of combining cell-penetrating sequences. The main advantage is that the target sequence is incubated with living cells instead of cell lysates and therefore the interactions will occur within the cellular context.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved