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University of Oklahoma Health Science Center

2 ARTICLES PUBLISHED IN JoVE

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JoVE Journal

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
Yuhui Chen 1, Xianfu Wang 2, Shunfei Lu 3, Hongcheng Wang 2, Shibo Li 2, Rujin Chen 1
1Laboratory of Plant Genetics and Development, Noble Research Institute, 2Genetics Laboratory, University of Oklahoma Health Science Center, 3Medicine and Health School, Li Shui University

This protocol provides experimental steps and information about reagents, equipment, and analysis tools for researchers who are interested in carrying out whole genome array-based comparative genomic hybridization (CGH) analysis of copy number variations in plants.

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Immunology and Infection

Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
Sunitha Pulikkot 1, Meng Zhao 2,3, Zhichao Fan 1
1Department of Immunology, School of Medicine, UConn Health, 2Arthritis and Clinical Immunology Program, Oklahoma Medical Research Foundation, 3Department of Microbiology and Immunology, University of Oklahoma Health Science Center

We describe stepwise protocols measuring the mitochondrial respiration of mouse and human neutrophils and HL60 cells using the metabolic extracellular flux analyzer.

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