LabEx IGO, "Immunotherapy, Graft, Oncology"

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

Marie-Claire Devilder^1,2,3, Mélinda Moyon^1,2,3, Xavier Saulquin^1,2, Laetitia Gautreau-Rolland^1,2

¹CRCINA, Inserm, CNRS, Université d'Angers, Université de Nantes, ²LabEx IGO, "Immunotherapy, Graft, Oncology", ³CHU Nantes, France

We describe a method for the production of human antibodies specific for an antigen of interest, starting from rare B cells circulating in human blood. Generation of these natural antibodies is efficient and rapid, and the antibodies obtained can discriminate between highly related antigens.