State Key Laboratory of Oncogenes and Related Genes
2 ARTICLES PUBLISHED IN JoVE
Bioengineering
Visual Detection of Multiple Nucleic Acids in a Capillary Array
Jianwei Chen *1,2,3, Ning Shao *1,2,3, Jiaying Hu *4, Rong Li 4, Yuanshou Zhu 1,2,3, Dabing Zhang 4,5, Shujuan Guo 1,2,3, Junhou Hui 6, Peng Liu 6, Litao Yang 4, Sheng-Ce Tao 1,2,3
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering, Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University
This protocol describes the fabrication of a small, ready-to-use cassette that can be applied for visual detection of multiple nucleic acids in a single, test that is easy to operate. In this approach, a capillary array was used for multiplex and highly efficient detection of GMO targets.
Cancer Research
A Surgical Procedure for Thyroidectomy: Para-Tracheal Capsular Dissection and its Benefit in the Protection of the Parathyroid Gland
Jian-hua Gu *1, Yan-na Zhao *2, Yiu-Sing Tsang *3, Guohui Xiao 3, Rong-li Xie 4, Wen-juan Xu 1, Jianming Yuan 4, Zhi-feng Zhao 4, Zi-huai Nie 1, Min Ding 4, Li Huang 5, Dong-jie Shen 4, Jian Fei 3,6,7,8,9
1Department of General Surgery, Integrated Traditional and Western Medicine Hospital of Huangpu District, 2Department of Ultrasound, Shanghai Tongji Hospital, 3Department of General Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 4Department of Surgery, Luwan Branch, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 5Department of Ultrasound, Shanghai Ruijin Rehabilitation Hospital, 6Department of General Surgery, Pancreatic Disease Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 7Research Institute of Pancreatic Diseases, Shanghai Jiao Tong University School of Medicine, 8State Key Laboratory of Oncogenes and Related Genes, 9Institute of Translational Medicine, Shanghai Jiao Tong University
Para-tracheal capsular dissection in thyroidectomy better preserves parathyroid function than the conventional method, shown by higher postoperative parathyroid hormone (PTH) levels and fewer cases of hypoparathyroidism without affecting calcium levels, suggesting its superiority in safeguarding parathyroid health. Here, we present a step-by-step protocol describing para-tracheal capsular dissection.