California State University, Chico

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos

A.C. Berrun¹, D.L. Stachura¹

¹Department of Biological Sciences, California State University, Chico

Here, we present a simple method to quantitate hematopoietic stem and progenitor cells (HSPCs) in embryonic zebrafish. HSPCs from dissociated zebrafish are plated in methylcellulose with supportive factors, differentiating into mature blood. This allows the detection of blood defects and allows drug screening to be easily conducted.

Developmental Biology

Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish

Kristen F. Rueb¹, David L. Stachura¹

¹Department of Biological Sciences, California State University, Chico

This assay is a simple method to quantitate hematopoietic cells in developing embryonic zebrafish. Blood cells from dissociated zebrafish are subjected to flow cytometry analysis. This allows the detection of blood defects in mutant animals and after genetic modification.