JoVE Logo

Sign In

The University of Alabama

2 ARTICLES PUBLISHED IN JoVE

image

Genetics

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Saman Saeidi *1, Michael R. McKain *1,2, Elizabeth A. Kellogg 1
1Donald Danforth Plant Science Center, 2Department of Biological Sciences, The University of Alabama

This article demonstrates a detailed protocol for DNA isolation and high-throughput sequencing library construction from herbarium material including rescue of exceptionally poor-quality DNA.

image

Biochemistry

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B
Zekiye Ceren Arituluk 1,2, Bishnu Adhikari 1, Urmila Maitra 1, Caroline Goodman 1, Lukasz M. Ciesla 1
1Department of Biological Sciences, The University of Alabama, 2Department of Pharmaceutical Botany, Faculty of Pharmacy, Hacettepe University

The protocol describes the preparation of cell membrane affinity chromatography (CMAC) columns with immobilized cell membrane fragments containing functional transmembrane tropomyosin kinase receptor B proteins. The use of CMAC columns in the identification of specialized plant metabolites interacting with these receptors and present in complex natural mixtures is also explained.

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved