The Scripps Research Institute Center on Aging

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans

Mark Lucanic¹, Theo Garrett¹, Matthew S. Gill², Gordon J. Lithgow¹

¹The Buck Institute for Research on Aging, ²The Scripps Research Institute Center on Aging

Here we describe a simple protocol for rapidly producing hundreds of nematode growth media agar, 96-well culture plates with consistent numbers of Caenorhhabditis elegans per well. These cultures are useful for the phenotypic screening of whole organisms. We focus here on using these cultures to screen chemicals for pro-longevity effects.