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University of Miami School of Medicine

3 ARTICLES PUBLISHED IN JoVE

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Neuroscience

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans
Bhavya Ravi *1, Layla M. Nassar *1,2, Richard J. Kopchock III *2, Pravat Dhakal *2, Michael Scheetz 2, Kevin M. Collins 1,2
1Neuroscience Program, University of Miami School of Medicine, 2Department of Biology, University of Miami

This protocol describes the use of genetically encoded Ca2+ reporters to record changes in neural activity in behaving Caenorhabditis elegans worms.  

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Cancer Research

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
Hongwei Shao 1, Mecker Moller 1, Dazhi Wang 1, Albert Ting 1, Marcia Boulina 2, Zhao-Jun Liu 1
1Department of Surgery, University of Miami School of Medicine, 2Analytical Imaging Core Facility, University of Miami School of Medicine

A novel three-dimensional spheroid model based on the heterotypic interaction of tumor cells and stromal fibroblasts is established. Here, we present coculture of tumor cells and stromal fibroblasts, time-lapse imaging, and confocal microscopy to visualize the formation of spheroids. This three-dimensional model offers a pertinent platform to study tumor-stroma interactions and test cancer therapeutics.

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Medicine

High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model
Antoine J. Ribieras 1, Yulexi Y. Ortiz 1,2, Sophie Shrestha 3, C. Theodore Huerta 1, Hongwei Shao 1,2, Maria E. Boulina 4, Roberto I. Vazquez-Padron 1,2, Zhao-Jun Liu 1,2, Omaida C. Velazquez 1,2
1Dewitt Daughtry Family Department of Surgery, University of Miami Miller School of Medicine, 2Vascular Biology Institute, University of Miami Miller School of Medicine, 3University of Miami, 4Analytical Imaging Core Facility, University of Miami

The present protocol describes a unique, clinically relevant model of peripheral arterial disease that combines femoral artery and vein electrocoagulation with the administration of a nitric oxide synthase inhibitor to induce hindlimb gangrene in FVB mice. Intracardiac DiI perfusion is then used for high-resolution, three-dimensional imaging of the footpad vasculature.

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