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Université PSL, CNRS

1 ARTICLES PUBLISHED IN JoVE

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Neuroscience

High-resolution Volume Imaging of Neurons by the Use of Fluorescence eXclusion Method and Dedicated Microfluidic Devices
Céline Braïni 1, Angelo Mottolese 1, Ivan Ferrante 1, Sylvain Monnier 2, Catherine Villard 1
1Laboratoire Physico-Chimie Curie, Institut Curie, Institut Pierre-Gilles de Gennes pour la microfluidique, Université PSL, CNRS, 2UMR 144 Institut Curie, Université PSL, CNRS

Volume is an important parameter regarding physiological and pathological characteristics of cells. We describe a fluorescent exclusion method allowing full-field measurement of in vitro neuronal volume with sub-micrometric axial resolution required for the analysis of neurites and dynamic structures implied in neuronal growth.

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