Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental ToxicantsSophia Houari 1,2, Sylvie Babajko 1,2, Sophia Loiodice 1,2, Ariane Berdal 1,2, Katia Jedeon 1,2
1Institut National de la Santé et Recherche Médicale (INSERM) UMRS 1138, Paris-Diderot University, Pierre & Marie Curie University, Paris-Descartes University, Laboratory of Molecular Oral Pathophysiology, Cordeliers Research Centre, 2Unit of Formation and Research (UFR) of Odontology, Paris-Diderot University
Understanding enamel formation and possible alterations requires the study of ameloblast activity. Here, we describe a reliable and consistent method to micro-dissect enamel organs containing secretion- and maturation-stage ameloblasts that may be used for further quantitative and qualitative experimental procedures.