Monash Biomedicine Discovery Institute

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain

Mayra A. Machuca^1,2, Anna Roujeinikova^1,2,3

¹Infection and Immunity Program, Monash Biomedicine Discovery Institute, ²Department of Microbiology, Monash University, ³Department of Biochemistry and Molecular Biology, Monash University

A procedure is presented for the refolding of the dCACHE periplasmic ligand binding domain of Campylobacter jejuni chemoreceptor Tlp3 from inclusion bodies and the purification to yield milligram quantities of protein.