Erasmus MC University Medical Center

3 ARTICLES PUBLISHED IN JoVE

Cancer Research

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Simone Punt¹, Robert J. Baatenburg de Jong², Ekaterina S. Jordanova^1,3

¹Department of Pathology, Leiden University Medical Center, ²Department of Otorhinolaryngology and Head and Neck Surgery, Erasmus University Medical Center, ³Center for Gynecological Oncology Amsterdam, VU Medical Center

Multiparameter fluorescence immunohistochemistry can be used to assess the number, relative distribution, and localization of immune cell populations in the tumor microenvironment. This manuscript describes the use of this technique to analyze T-cell subpopulations in oropharyngeal cancer.

Medicine

Transplantation of Adipose Tissue-Derived Stem Cell Sheet to Reduce Leakage After Partial Colectomy in A Rat Model

Panithi Sukho^1,2,3, Geesien S.A. Boersema⁴, Nicole Kops⁵, Johan F. Lange⁴, Jolle Kirpensteijn^1,6, Jan Willem Hesselink¹, Yvonne M. Bastiaansen-Jenniskens⁵, Femke Verseijden^1,5

¹Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, ²Department of Otorhinolaryngology, Erasmus MC University Medical Center, ³Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, ⁴Department of Surgery, Erasmus MC University Medical Center, ⁵Department of Orthopaedics, Erasmus MC University Medical Center, ⁶Hill's Pet Nutrition Inc.

The preparation and transplantation of adipose tissue derived stem cell (ASC) sheets onto insufficiently sutured colorectal anastomoses in a rat model is presented. This novel application shows that ASC sheets can reduce colorectal anastomosis leakage.

Medicine

Intraoperative Assessment of Resection Margins in Oral Cavity Cancer: This is the Way

Yassine Aaboubout^1,2, Elisa M. Barroso^1,3,4, Mahesh Algoe¹, Patricia C. Ewing-Graham¹, Ivo ten Hove^3,6, Hetty Mast³, José A. Hardillo², Aniel Sewnaik², Dominiek A. Monserez², Stijn Keereweer², Brend P. Jonker³, Cornelia G. F. van Lanschot², Roeland W. H. Smits², Maria R. Nunes Soares^1,4, Lars Ottevanger¹, Sanne E. Matlung¹, Paul A. Seegers⁵, Vera van Dis¹, Robert M. Verdijk¹, Eppo B. Wolvius³, Peter J. Caspers⁴, Tom C. Bakker Schut⁴, Robert J. Baatenburg de Jong², Gerwin J. Puppels⁴, Senada Koljenović¹

¹Department of Pathology, Erasmus MC University Medical Center, ²Department of Otorhinolaryngology and Head and Neck Surgery, Erasmus MC University Medical Center, ³Department of Oral and Maxillofacial Surgery, Erasmus MC University Medical Center, ⁴Department of Dermatology, Erasmus MC University Medical Center, ⁵PALGA foundation, The nationwide network and registry of histo- and cytopathology, ⁶Department of Oral and Maxillofacial Surgery, Leiden University Medical Center

The goal of this protocol is to provide a clear overview of specimen-driven intraoperative assessment of resection margins. It is encouraged to implement this protocol to improve patient care at other institutes.