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Indian Institute of Science

6 ARTICLES PUBLISHED IN JoVE

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Neuroscience

An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival
Zhongshu Tang 1, Shuihua Zhang 1,2, Chunsik Lee 1, Anil Kumar 1, Pachiappan Arjunan 1, Yang Li 1, Fan Zhang 1, Xuri Li 1
1National Eye Institute, NIH, 2Ophthalmology Department, The Second Hospital of Harbin Medical University

This protocol shows how to retrogradely label retinal ganglion cells, and how to subsequently make an optic nerve crush injury in order to analyze retinal ganglion cell survival and apoptosis. It is an experimental disease model for different types of optic neuropathy, including glaucoma.

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Medicine

A Mouse Model of the Cornea Pocket Assay for Angiogenesis Study
Zhongshu Tang 1, Fan Zhang 1, Yang Li 1, Pachiappan Arjunan 1, Anil Kumar 1, Chunsik Lee 1, Xuri Li 1
1National Eye Institute

The cornea is unique in that it lacks vascular tissues. However, robust blood vessel growth and survival can be induced in the cornea by potent angiogenic factors. Therefore, the cornea can provide with us a valuable tool for angiogenic studies. This protocol demonstrates how to perform the mouse model of cornea pocket assay and how to assess the angiogenesis induced by angiogenic factors using this model.

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Engineering

A Fabrication and Measurement Method for a Flexible Ferroelectric Element Based on Van Der Waals Heteroepitaxy
Jie Jiang *1, Yugandhar Bitla *2, Qiang-xiang Peng 1, Yi-Chun Zhou 1, Ying-Hao Chu 3
1Key Laboratory of Low Dimensional Materials and Application Technology of Ministry of Education, Xiangtan University, 2Department of Physics, Indian Institute of Science, 3Department of Materials Science and Engineering, National Chiao Tung University

In this paper, we present a protocol to directly grow an epitaxial yet flexible lead zirconium titanate memory element on muscovite mica.

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Immunology and Infection

User-friendly, High-throughput, and Fully Automated Data Acquisition Software for Single-particle Cryo-electron Microscopy
Anil Kumar 1, Surekha P. 1, Sahil Gulati 2, Somnath Dutta 1
1Molecular Biophysics Unit, Indian Institute of Science, 2Gatan Inc.

Single-particle cryo-electron microscopy demands a suitable software package and user-friendly pipeline for high-throughput automatic data acquisition. Here, we present the application of a fully automated image acquisition software package, Latitude-S, and a practical pipeline for data collection of vitrified biomolecules under low-dose conditions.

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Immunology and Infection

Characterizing Salmonella Typhimurium-induced Septic Peritonitis in Mice
Avik Chattopadhyay 1, Joel P. Joseph 2, Sai Shyam 3, Dipankar Nandi 1
1Department of Biochemistry, Indian Institute of Science, 2Center for BioSystems Science and Engineering, Indian Institute of Science, 3Undergraduate Program, Indian Institute of Science

This protocol describes the induction of Gram-negative monobacterial sepsis in a mouse model system. The model is useful in investigating the inflammatory and lethal host responses during sepsis.

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Biochemistry

Single-Molecule Diffusion and Assembly on Polymer-Crowded Lipid Membranes
Satyaghosh Maurya 1, Vishwesh Haricharan Rai 1, Aditya Upasani 1, Saurabh Umrao 1,2, Diksha Parwana 1, Rahul Roy 1,3
1Department of Chemical Engineering, Indian Institute of Science, 2Holonyak Micro & Nanotechnology Lab, University of Illinois, Urbana-Champaign, 3Center for BioSystems Science and Engineering, Indian Institute of Science

Here, a protocol to perform and analyze the binding, mobility, and assembly of single molecules on artificial crowded lipid membranes using single-molecule total internal reflection fluorescence (smTIRF) microscopy is presented.

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