Leibniz Institute for Molecular Pharmacology (FMP)/ NeuroCure Cluster of Excellence

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice

Franziska Bender^1,2, Tatiana Korotkova^2,3, Alexey Ponomarenko^1,2

¹Systems Neurophysiology Research Group, Institute of Clinical Neuroscience and Medical Psychology, Medical Faculty, Heinrich Heine University Düsseldorf, ²Behavioural Neurodynamics Group, Leibniz Institute for Molecular Pharmacology (FMP)/ NeuroCure Cluster of Excellence, ³Neuronal Circuits and Behavior Research Group, Max Planck Institute for Metabolism Research

We describe the use of optogenetics and electrophysiological recordings for selective manipulations of hippocampal theta oscillations (5-10 Hz) in behaving mice. The efficacy of the rhythm entrainment is monitored using local field potential. A combination of opto- and pharmacogenetic inhibition addresses the efferent readout of hippocampal synchronization.