Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of TissueChloé J Simard *1, Guillaume Pelletier *1, Luc H Boudreau 1, Etienne Hebert-Chatelain 2, Nicolas Pichaud 1
1Département de chimie et biochimie, Université de Moncton, 2Département de biologie, Université de Moncton
In this paper, a method to measure oxygen consumption using high-resolution respirometry in permeabilized thoraxes of Drosophila is described. This technique requires a minimal amount of tissue compared to the classic mitochondrial isolation technique and the results obtained are more physiologically relevant.
