Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor XenograftsTina T. Thomas 1, Sahiti Chukkapalli 1, Raelene A. Van Noord 1, Melanie Krook 2, Mark J. Hoenerhoff 3, Jonathan R. Dillman 4, Elizabeth R. Lawlor 2,5, Valerie P. Opipari 5, Erika A. Newman 1
1Departments of Surgery, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School, 2Departments of Pathology, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School, 3Unit for Laboratory Animal Medicine, The University of Michigan Medical School, 4Departments of Radiology, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School, 5Departments of Pediatrics, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School
Here, we present a protocol to utilize ultrasound-guided injection of neuroblastoma (NB) and Ewing's sarcoma (ES) cells (established cell lines and patient-derived tumor cells) at biologically relevant sites to create reliable preclinical models for cancer research.
