The First Affiliated Hospital of Guangxi Medical University

2 ARTICLES PUBLISHED IN JoVE

Biology

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)

Zhen Wang¹, Christoph Benning¹

¹Department of Biochemistry and Molecular Biology, Michigan State University

Composition of polar lipid extracts and the fatty acid composition of individual glycerolipids are determined in a simple and robust lipid profiling experiment. For this purpose, glycerolipids are isolated by thin layer chromatography and subjected to transmethylation of their acyl groups. Fatty acyl methylesters are quantified by gas-liquid chromatography.

Biology

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse

Zhen Wang^1,4, Ramon Ocadiz-Ruiz¹, Sinju Sundaresan¹, Lin Ding¹, Michael Hayes¹, Nirakar Sahoo³, Haoxing Xu^1,2, Juanita L. Merchant^1,2,5

¹Department of Internal Medicine-Gastroenterology, University of Michigan, ²Department of Molecular and Integrative Physiology, University of Michigan, ³Department of Molecular, Cellular and Developmental Biology, University of Michigan, ⁴Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, ⁵Division of Gastroenterology, University of Arizona College of Medicine

Here, we describe the isolation of enteric-glial cells from the intestinal-submucosa using sequential EDTA incubations to chelate divalent cations and then incubation in non-enzymatic cell recovery solution. Plating the resultant cell suspension on poly-D-lysine and laminin results in a highly enriched culture of submucosal glial cells for functional analysis.