Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell ProtrusionsAnaïs Bouissou *1, Amsha Proag *1, Marion Portes 1, Vanessa Soldan 2, Stéphanie Balor 2, Christophe Thibault 3,4, Christophe Vieu 3,4, Isabelle Maridonneau-Parini 1, Renaud Poincloux 1
1Institut de Pharmacologie et Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, 2METi, 3CNRS, LAAS, 4Univ de Toulouse, INSA
Here, we detail the experimental techniques used to evaluate the protrusion forces that podosomes apply on a compliant film, from the preparation of the film to the automated analysis of topographical images.
