Singapore General Hospital

3 ARTICLES PUBLISHED IN JoVE

Biology

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

¹Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences

We describe a fluorescence reporter assay to quickly identify and characterize genes that regulate mouse embryonic stem cell maintenance and self-renewal.

Medicine

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

Midhat H. Abdulreda^1,2, Alejandro Caicedo^1,3,4, Per-Olof Berggren^1,5

¹Diabetes Research Institute, University of Miami Miller School of Medicine, ²Department of Surgery, University of Miami Miller School of Medicine, ³Department of Medicine, University of Miami Miller School of Medicine, ⁴Department of Physiology & Biophysics, University of Miami Miller School of Medicine, ⁵The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet

A new approach combining intraocular transplantation and confocal microscopy enables longitudinal, non-invasive real-time imaging with single-cell resolution within grafted tissues in vivo. We demonstrate how to transplant pancreatic islets into the anterior chamber of the mouse eye.

Biology

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

Xiaofeng Zheng^1,2, Calvin Qing Wei Ho¹, Xiaowei Zheng³, Kian Leong Lee⁴, Katarina Gradin⁵, Teresa S. Pereira³, Per-Olof Berggren^1,2,3, Yusuf Ali^1,2

¹Lee Kong Chian School of Medicine, Nanyang Technological University, ²Singapore Eye Research Institute (SERI), Singapore General Hospital, ³The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, ⁴Cancer and Stem Cell Biology Program, Duke-NUS Medical School, ⁵Department of Cell and Molecular Biology, Karolinska Institutet

Here we describe a co-immunoprecipitation protocol to study protein-protein interactions between endogenous nuclear proteins under hypoxic conditions. This method is suitable for demonstration of the interactions between transcription factors and transcriptional co-regulators at hypoxia.